Similar products: Activated thiol Sepharose, activated thiol agarose, thiopropyl Sepharose 6B, thiopropyl activated agarose.

Applications: acyl-RAC protein assays; Protein Palmitoylation; S-Palmitoylation; Palmitoylating; Site-specific protein immobilization.

Description:

High capacity acyl-rac thiol capture,S3 Beads are one of Nanocs reactive agarose beads that can be used for reversible immobilization of biomolecules containing free thiol groups. S3^TM  thiol capture beads contain sulfhydryl reactive bonds that can react easily with free thiol groups under mild conditions. Reaction between thiolated molecules and S3 resins results a reversible dithio bond that can be cleaved by common dithio reducing agents such as DTT or TCEP. Compared to other thiol reactive beads, Nanocs S3^TM Beads have exceptionally high thiol capture capacity; Density of thiol reactive groups on S3 beads is 20-30 times more over conventional thiol activated agarose beads. S3 capture beads can be used in many applications, such as acyl-RAC protein assays; Protein S-palmitolytation; Site-specific protein immobilizations;  Protein purification, digestion and analysis. S3 beads are ideal replacements for commonly used activated Thiol-Sepharose 4B or Thiopropyl Sepharose 6B from GE.

Product Features:

• High thiol binding capacity;
• Superior thiol reactivity:
• Reversible disulfide linkage;
• Low non-specific binding;

Product Specification:

• Bead Matrix: 6B pegylated agarose beads;
• Bead Size: 50~150 microns;
• Ligand type: Thiol reactive ligands;
• Ligand density: 800-1000 umol/g  beads;
• Reactive to: Free sulfhydryl/thiol groups (cystein, etc.);
• Form: Lyophilized powder.

Storage Conditions:

• Store at 4⁰C or lower.

Acyl-rac Related Reagents:

• DTT (Dithiothreitol)
• TCEP.HCl
• Hydroxylamine
• Ellman's reagent
• mPEG Maleimide

References:

Motipally, S. I., Myers, B., Sechrest, E. R., Sokolov, D., Murphy, J. and Kolandaivelu, S. (2023). A Modified Acyl-RAC Method of Isolating Retinal Palmitoyl Proteome for Subsequent Detection through LC-MS/MS. Bio-protocol 13(8): e4654. DOI: 10.21769/BioProtoc.4654.