Synonyms: Silane PEG600 NHS, NHS PEG600 Silane, Silane PEG NHS, MW 600 Da

Description:

Silane PEG600 NHS is one of Nanocs's surface reactive PEG derviatives that has a short PEG600 chain and an amine reactive NHS group. Ethoxy or methoxyl silane functionalized polyethylene glycol, silane PEG (PEG-Si) is a surface reactive PEG derivative that can be used to modify glass, silica and other surfaces via the reaction between hydroxyl group and ethoxyl/methoxyl silane. NHS, on the other hand, can react with primary amine group rapidly under alkaline conditions (pH 8-10) to form a stable amide bond. Pegylation can greatly suppress the non-specific binding of charged molecules to the modified surfaces. And they have wide applications for medica device, biomems or biocompatible material development.

Physical Properties:

• Form: Semi-solid;
• Color: Pale yellow to amber;
• PEG type: linear PEG, MW 600 Da, 12~15 PEG units;
• Silane type: Triethoxyl silane;
• Reactive group: Succinimidyl ester, NHS;
• Reactive to: Glass/silicon surfaces and primary amines;
• Purity: >90%.

Storage Conditions:

• Store at -20 ⁰C, desiccated. Silane PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, silane PEG is first dissolved in a mixture of ethanol/water solution, then allow the solution to contact with oxidized glass or silica surface for 30 min to 2 hours. Under this condition, silane PEG should bind to the material covalently.

Materials Required:

• Pegylation solution: Ethanol/water (w/w, 95%/5%);
• Silane PEG stock solution, 10~50 mg/mL in DMSO, prepare in fresh.
• Washing solution: Distilled water.

Reaction Steps:

1. Dissolve targeted materials in Pegylation buffer.

2. Add silane PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of silane PEG needed for optimal conjugation;

3. Allow mixture agitates at room temperature for 30 min at room temperature or 2 hours at 4 -20 ⁰C.

4. Wash out unreacted materials either by distilled water.