DBCO PEG amine, MW 1000, 2000, 3400, 5000, 10000 Da:

Nanocs’ DBCO (dibenzocyclooctyne) PEG amine is a heterobifunctional reactive PEG derivative that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneously without the need for toxic Cu catalysts. Amine groups, on the other hand, can react with a variety of functional groups such as COOH, NHS, aldehyde, etc.

Nanocs DBCO PEG amine features:

• Appearance: Off-white solid or semi-solid depends on MW of PEG;
• Solubility: Soluble in water, DMSO and chloroform;
• Reactivity: DBCO reactive to azide group while amine reactive to COOH, NHS, aldehyde and many other groups.

Copper-free Click reaction procedures:

• Prepare the azide-containing reagents in reaction buffer.
• Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
• Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 ⁰C.
• Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage Conditions:

• Store at -20 ⁰C, desiccate. Protect from light. Avoid frequent thaw and freeze.